Preventing Proteolytic Artifacts in the Baculovirus Expression System
نویسندگان
چکیده
منابع مشابه
Preventing proteolytic artifacts in the baculovirus expression system.
The baculovirus expression system has become established as a popular method for obtaining high levels of recombinant protein synthesis in a eukaryotic environment (5). In a number of cases, however, researchers have reported decreased product quality and yield resulting from proteolytic activity late in the course of infection (2,3,8,12). The enzyme most likely to cause this degradation is V-C...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98251bm01